Frozen tissues samples have been widely used in medical research for cancer and other diseases to examine cell morphology, disease status, intracellular and intercellular communication, as well as biopsy tissue sections.
Compared to FFPE sample preparation, freezing has the advantage of being fast, and the resulting samples are very suitable for molecular analysis. Frozen tissue samples are also preferred in analysis such as mass spectrometry, next generation sequencing, western blotting for post-translational protein modifications, and quantitative real-time polymerase chain reaction. Therefore, frozen tissue samples are the gold standard for DNA and RNA sequencing, especially when the strands must be longer than 50 pairs. In immunohistochemistry, frozen tissue samples are also very useful as the proteins are preserved in a native state.
At Creative Bioarray, all fresh frozen tissue samples are collected by certified medical pathologists. The small pieces of tissue are snap-frozen in liquid nitrogen usually within 20-30 minutes after the surgical excision. Standard sample weight is 0.5-1.0 gram. An embedding media can be added upon request.
Fresh Frozen Tissue Collection Formats
Our fresh frozen tissue specimens are offered in several formats to meet the needs of biomedical research:
- Individual snap-frozen tissue samples, diseased or normal
- Fresh frozen diseased and normal adjacent tissues from a single donor
- Paired fresh frozen and formalin-fixed paraffin-embedded (FFPE) tissues from a single donor
- Fresh frozen diseased and metastatic tissue samples from a single donor
- Fresh frozen diseased or normal tissues with matched blood products or biofluids
Fresh Frozen Tissue Specimens Currently in Our Inventory
- Frozen Tissue Blocks
- Frozen Tissue Sections
Our tissue samples are processed consistently according to validated protocols. Each tissue sample is snap-frozen to preserve mRNA and protein, and is matched with unaffected human tissue, as well as DNA and sera from the donor in most cases. To inquire about the current availability of specimens or to investigate the feasibility of your custom project, contact our office or send us an inquiry via email.