The isolation of nuclear, cytosolic and mitochondrial fractions of a certain purity from mammalian tissues and cells has attracted great interest due to their advantages in the study and characterization of different cellular proteins and organelles. These subcellular fractions are commercially available from individual livers (inter-individual variation can be studied) or a pool of livers (representative of the average enzyme activities of the population). They can be stored and remain stable at -80°C for many years and can be thawed easily. They are very easy to handle for several types of incubations and they are suitable for full automation. All of these advantages make them very useful for metabolism studies in the early stage of drug development including the screening program.
Subcellular fractionation utilizes one or more of the properties of each compartment, such as buoyant density, surface charge density, size, and shape, and is mainly based on differential centrifugation in a high viscosity media. Gel filtration, affinity chromatography, electrophoresis or selective density-shift perturbation can also be used. Variations in the conditions of the available protocols are dependent on the organelle, tissue or cell type and equipment used.
- Fractionation by centrifugation
- Analysis of fractions
Creative Bioarray offers a variety of options when it comes to subcellular fractions. Our subcellular fractions are produced from fresh tissues to ensure the highest levels of enzyme activity.
Custom subcellular fractions can be easily ordered to meet the specific needs of any study. Contact our dedicated product specialists to find out how Creative Bioarray's custom products can meet your needs.