FFPE Tissue Protein Extraction Kit
Product ID: HPE4
Protein Extraction
| OVERVIEW | |
|---|---|
| The FFPE Tissue Protein Extraction Kit uses a proprietary decrosslinking technology to extract proteins from formalin-fixed paraffin-embedded (FFPE) specimens. It combines selective chelating agents and recombinant proteases to reverse fixation-induced crosslinks, recovering full-length proteins with preserved epitopes and post-translational modifications. The kit replaces harsh alkaline hydrolysis or prolonged heating with pH-modulated enzymatic digestion under non-denaturing conditions, maintaining protein functionality. The workflow yields extracts suitable for high-sensitivity analyses like MS/MS, RPPA, and multiplex immunoassays. | |
| Product Details | |
| Sample Type | FFPE Tissue |
| Sample size | 2–3 x 100 mm2 per prep |
| Size | 1 kit |
| Yeild | 20–80 µg |
| Applications | SDS-PAGE; Western Blot; Mass Spectrometry |
| LIMITATIONS | |
| All products are for research use only. | |
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